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Hector Viadiu
LABORATORY OF STRUCTURAL BIOCHEMISTRY:
X-ray Crystallography and Cryo-Electron Microscopy of
DNA Binding Proteins and Membrane Proteins
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| Contact Information |
| Assistant Professor |
| Office: NSB 4316 |
| Phone: (858) 2-4486 |
| Fax: (858) 822--449 |
| Email: viadiu@ucsd.edu |
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| View group members
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| Education and Appointments |
| 2000 |
Ph.D., Columbia University
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| Awards and Academic Honors |
| 2008 |
Hellman Award |
| 2001-2006 |
Postdoctoral Fellow, Harvard Medical School |
| 2001-2003 |
Damon-Runyon Fellow |
| 1993-1998 |
Fulbright-CONACYT Fellow |
| Research Interests |
Our program on Structural Biochemistry integrates high-resolution information obtained by X-ray Crystallography with Cryo-Electron Microscopy data to study DNA-binding proteins and membrane proteins.
In the past, we have studied the catalysis and specificity of the restriction endonuclease BamHI to atomic resolution by X-ray crystallography, the specificity of Aquaporin-9 by electron crystallography and the specificity of beta-lactamase by combinatorial mutagenesis. We have also used single particle reconstruction techniques to obtain a 25 Å structure of the complex separase-securin.
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| Primary Research Area: |
Interdisciplinary Specialties: |
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Biochemistry
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Macromolecular Structure
Biophysics
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| Image Gallery: |
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Figure 1: X-RAY CRYSTALLOGRAPHY: Structure of the Restriction Endonuclease BamHI bound to Non-Specific DNA. |
Figure 2: ELECTRON CRYSTALLOGRAPHY: Structure of Aquaporin-9. |
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Figure 3: ELECTRON MICROSCOPY-SINGLE PARTICLE RECONSTRUCTION: Structure of the Complex Separase-Securin
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| Selected Publications |
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Viadiu H,
"Molecular architecture of tumor suppressor p53." Curr Top Med Chem 15(1327-34):
, 2008. [Go to PubMed]
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Viadiu H,
Gonen T,
Walz T,
"Projection map of aquaporin-9 at 7 A resolution." J Mol Biol 1(80-8):
, 2007. [Go to PubMed]
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Vanamee ES,
Viadiu H,
Kucera R,
Dorner L,
Picone S,
Schildkraut I,
Aggarwal AK,
"A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA." EMBO J 23(4198-208):
, 2005. [Go to PubMed]
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Viadiu H,
Stemmann O,
Kirschner MW,
Walz T,
"Domain structure of separase and its binding to securin as determined by EM." Nat Struct Mol Biol 6(552-3):
, 2005. [Go to PubMed]
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Viadiu H,
Vanamee ES,
Jacobson EM,
Schildkraut I,
Aggarwal AK,
"Crystallization of restriction endonuclease SfiI in complex with DNA." Acta Crystallogr D Biol Crystallogr Pt 8(1493-5):
, 2003. [Go to PubMed]
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Scully KM,
Jacobson EM,
Jepsen K,
Lunyak V,
Viadiu H,
Carrière C,
Rose DW,
Hooshmand F,
Aggarwal AK,
Rosenfeld MG,
"Allosteric effects of Pit-1 DNA sites on long-term repression in cell type specification." Science 5494(1127-31):
, 2000. [Go to PubMed]
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Viadiu H,
Aggarwal AK,
"Structure of BamHI bound to nonspecific DNA: a model for DNA sliding." Mol Cell 5(889-95):
, 2000. [Go to PubMed]
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Viadiu H,
Kucera R,
Schildkraut I,
Aggarwal AK,
"Crystallization of restriction endonuclease BamHI with nonspecific DNA." J Struct Biol 1(81-5):
, 2000. [Go to PubMed]
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Viadiu H,
Aggarwal AK,
"The role of metals in catalysis by the restriction endonuclease BamHI." Nat Struct Biol 10(910-6):
, 1998. [Go to PubMed]
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Viadiu H,
Osuna J,
Fink AL,
Soberón X,
"A new TEM beta-lactamase double mutant with broadened specificity reveals substrate-dependent functional interactions." J Biol Chem 2(781-7):
, 1995. [Go to PubMed]
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